wild type erdman m tuberculosis Search Results


middlebrook 7h9 medium  (MiddleBrook Pharmaceuticals)
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MiddleBrook Pharmaceuticals middlebrook 7h9 medium
Middlebrook 7h9 Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nc 022350 1 haarlem 4 nz cp010337 1  (ATCC)
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ATCC nc 022350 1 haarlem 4 nz cp010337 1
Nc 022350 1 Haarlem 4 Nz Cp010337 1, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mycobacterium tuberculosis erdman  (ATCC)
94
ATCC mycobacterium tuberculosis erdman
Sublingual immunization with <t>Mycobacterium</t> <t>tuberculosis</t> (Mtb) proteins Ag85B and ESAT-6 in the presence of alpha-galactosylceramide (α-GalCer) adjuvant induces systemic and mucosal T cell responses. Separate groups of mice ( n = 4–6) were immunized by sublingual route with one or two doses of mixture of Mtb proteins Ag85B and ESAT-6 in the presence or absence of α-GalCer adjuvant (w/aGC and w/o aGC, respectively) at 7 day intervals and sacrificed 7 days after each immunization to determine immune responses. Single cell suspensions from spleen and lung tissues were analyzed for IFN-γ producing cells specific for ESAT-6 (Panels ( A , B )) and Ag85B (Panels ( C , D )) using a standard IFN-γ ELISpot assay. Responses specific to the Mtb proteins were determined by subtracting the background values of medium stimulation from that of the individual protein stimulation and the mean ± S.D values were expressed as spot forming cells (SFC) per 10 5 input cells. Significant responses ( p < 0.05) between groups vaccinated in the presence and absence of α-GalCer (*) and between one and two doses (#) of vaccine delivered were noted. The experiment was performed using 5 mice/group and repeated 2 times.
Mycobacterium Tuberculosis Erdman, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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25618 m tuberculosis erdman  (ATCC)
96
ATCC 25618 m tuberculosis erdman
Sublingual immunization with <t>Mycobacterium</t> <t>tuberculosis</t> (Mtb) proteins Ag85B and ESAT-6 in the presence of alpha-galactosylceramide (α-GalCer) adjuvant induces systemic and mucosal T cell responses. Separate groups of mice ( n = 4–6) were immunized by sublingual route with one or two doses of mixture of Mtb proteins Ag85B and ESAT-6 in the presence or absence of α-GalCer adjuvant (w/aGC and w/o aGC, respectively) at 7 day intervals and sacrificed 7 days after each immunization to determine immune responses. Single cell suspensions from spleen and lung tissues were analyzed for IFN-γ producing cells specific for ESAT-6 (Panels ( A , B )) and Ag85B (Panels ( C , D )) using a standard IFN-γ ELISpot assay. Responses specific to the Mtb proteins were determined by subtracting the background values of medium stimulation from that of the individual protein stimulation and the mean ± S.D values were expressed as spot forming cells (SFC) per 10 5 input cells. Significant responses ( p < 0.05) between groups vaccinated in the presence and absence of α-GalCer (*) and between one and two doses (#) of vaccine delivered were noted. The experiment was performed using 5 mice/group and repeated 2 times.
25618 M Tuberculosis Erdman, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bacterial infection mycobacterium tuberculosis erdman  (ATCC)
93
ATCC bacterial infection mycobacterium tuberculosis erdman
Sublingual immunization with <t>Mycobacterium</t> <t>tuberculosis</t> (Mtb) proteins Ag85B and ESAT-6 in the presence of alpha-galactosylceramide (α-GalCer) adjuvant induces systemic and mucosal T cell responses. Separate groups of mice ( n = 4–6) were immunized by sublingual route with one or two doses of mixture of Mtb proteins Ag85B and ESAT-6 in the presence or absence of α-GalCer adjuvant (w/aGC and w/o aGC, respectively) at 7 day intervals and sacrificed 7 days after each immunization to determine immune responses. Single cell suspensions from spleen and lung tissues were analyzed for IFN-γ producing cells specific for ESAT-6 (Panels ( A , B )) and Ag85B (Panels ( C , D )) using a standard IFN-γ ELISpot assay. Responses specific to the Mtb proteins were determined by subtracting the background values of medium stimulation from that of the individual protein stimulation and the mean ± S.D values were expressed as spot forming cells (SFC) per 10 5 input cells. Significant responses ( p < 0.05) between groups vaccinated in the presence and absence of α-GalCer (*) and between one and two doses (#) of vaccine delivered were noted. The experiment was performed using 5 mice/group and repeated 2 times.
Bacterial Infection Mycobacterium Tuberculosis Erdman, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m tuberculosis 97 erdman  (ATCC)
92
ATCC m tuberculosis 97 erdman
Sublingual immunization with <t>Mycobacterium</t> <t>tuberculosis</t> (Mtb) proteins Ag85B and ESAT-6 in the presence of alpha-galactosylceramide (α-GalCer) adjuvant induces systemic and mucosal T cell responses. Separate groups of mice ( n = 4–6) were immunized by sublingual route with one or two doses of mixture of Mtb proteins Ag85B and ESAT-6 in the presence or absence of α-GalCer adjuvant (w/aGC and w/o aGC, respectively) at 7 day intervals and sacrificed 7 days after each immunization to determine immune responses. Single cell suspensions from spleen and lung tissues were analyzed for IFN-γ producing cells specific for ESAT-6 (Panels ( A , B )) and Ag85B (Panels ( C , D )) using a standard IFN-γ ELISpot assay. Responses specific to the Mtb proteins were determined by subtracting the background values of medium stimulation from that of the individual protein stimulation and the mean ± S.D values were expressed as spot forming cells (SFC) per 10 5 input cells. Significant responses ( p < 0.05) between groups vaccinated in the presence and absence of α-GalCer (*) and between one and two doses (#) of vaccine delivered were noted. The experiment was performed using 5 mice/group and repeated 2 times.
M Tuberculosis 97 Erdman, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m tuberculosis erdman  (ATCC)
90
ATCC m tuberculosis erdman
(A) Induction of autophagy in BMMs by ambroxol as determined by LC3B puncta. (B) Induction of autophagy in BMMs by ambroxol as determined by TFEB nuclear translocation. (C) Effects of ambroxol on M. <t>tuberculosis</t> <t>Erdman</t> survival in free culture and when infected into BMMs. Data are means ± SEM of 3 or 4 independent replicates. Tukey's multiple comparison: *, P < 0.05; **, P < 0.01; †, not significant. (D) Effect of ambroxol on autophagy in lung tissue. n = 2 mice/group. Data are means ± SEM.
M Tuberculosis Erdman, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m. tuberculosis strain erdman (bei resources-nr-15404 or atcc 35801  (BEI Resources)
90
BEI Resources m. tuberculosis strain erdman (bei resources-nr-15404 or atcc 35801
(A) Induction of autophagy in BMMs by ambroxol as determined by LC3B puncta. (B) Induction of autophagy in BMMs by ambroxol as determined by TFEB nuclear translocation. (C) Effects of ambroxol on M. <t>tuberculosis</t> <t>Erdman</t> survival in free culture and when infected into BMMs. Data are means ± SEM of 3 or 4 independent replicates. Tukey's multiple comparison: *, P < 0.05; **, P < 0.01; †, not significant. (D) Effect of ambroxol on autophagy in lung tissue. n = 2 mice/group. Data are means ± SEM.
M. Tuberculosis Strain Erdman (Bei Resources Nr 15404 Or Atcc 35801, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m. tuberculosis strain erdman (bei resources-nr-15404 or atcc 35801 - by Bioz Stars, 2026-10
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m tuberculosis hn878 ds 0 60 m tuberculosis erdman ds 1 56 m tuberculosis  (ATCC)
94
ATCC m tuberculosis hn878 ds 0 60 m tuberculosis erdman ds 1 56 m tuberculosis
(A) Induction of autophagy in BMMs by ambroxol as determined by LC3B puncta. (B) Induction of autophagy in BMMs by ambroxol as determined by TFEB nuclear translocation. (C) Effects of ambroxol on M. <t>tuberculosis</t> <t>Erdman</t> survival in free culture and when infected into BMMs. Data are means ± SEM of 3 or 4 independent replicates. Tukey's multiple comparison: *, P < 0.05; **, P < 0.01; †, not significant. (D) Effect of ambroxol on autophagy in lung tissue. n = 2 mice/group. Data are means ± SEM.
M Tuberculosis Hn878 Ds 0 60 M Tuberculosis Erdman Ds 1 56 M Tuberculosis, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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formycobacterium species mycobacterium species mic results  (ATCC)
98
ATCC formycobacterium species mycobacterium species mic results
(A) Induction of autophagy in BMMs by ambroxol as determined by LC3B puncta. (B) Induction of autophagy in BMMs by ambroxol as determined by TFEB nuclear translocation. (C) Effects of ambroxol on M. <t>tuberculosis</t> <t>Erdman</t> survival in free culture and when infected into BMMs. Data are means ± SEM of 3 or 4 independent replicates. Tukey's multiple comparison: *, P < 0.05; **, P < 0.01; †, not significant. (D) Effect of ambroxol on autophagy in lung tissue. n = 2 mice/group. Data are means ± SEM.
Formycobacterium Species Mycobacterium Species Mic Results, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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7h11 agar plates  (MiddleBrook Pharmaceuticals)
90
MiddleBrook Pharmaceuticals 7h11 agar plates
(A) Induction of autophagy in BMMs by ambroxol as determined by LC3B puncta. (B) Induction of autophagy in BMMs by ambroxol as determined by TFEB nuclear translocation. (C) Effects of ambroxol on M. <t>tuberculosis</t> <t>Erdman</t> survival in free culture and when infected into BMMs. Data are means ± SEM of 3 or 4 independent replicates. Tukey's multiple comparison: *, P < 0.05; **, P < 0.01; †, not significant. (D) Effect of ambroxol on autophagy in lung tissue. n = 2 mice/group. Data are means ± SEM.
7h11 Agar Plates, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Sublingual immunization with Mycobacterium tuberculosis (Mtb) proteins Ag85B and ESAT-6 in the presence of alpha-galactosylceramide (α-GalCer) adjuvant induces systemic and mucosal T cell responses. Separate groups of mice ( n = 4–6) were immunized by sublingual route with one or two doses of mixture of Mtb proteins Ag85B and ESAT-6 in the presence or absence of α-GalCer adjuvant (w/aGC and w/o aGC, respectively) at 7 day intervals and sacrificed 7 days after each immunization to determine immune responses. Single cell suspensions from spleen and lung tissues were analyzed for IFN-γ producing cells specific for ESAT-6 (Panels ( A , B )) and Ag85B (Panels ( C , D )) using a standard IFN-γ ELISpot assay. Responses specific to the Mtb proteins were determined by subtracting the background values of medium stimulation from that of the individual protein stimulation and the mean ± S.D values were expressed as spot forming cells (SFC) per 10 5 input cells. Significant responses ( p < 0.05) between groups vaccinated in the presence and absence of α-GalCer (*) and between one and two doses (#) of vaccine delivered were noted. The experiment was performed using 5 mice/group and repeated 2 times.

Journal: Vaccines

Article Title: Prophylactic Sublingual Immunization with Mycobacterium tuberculosis Subunit Vaccine Incorporating the Natural Killer T Cell Agonist Alpha-Galactosylceramide Enhances Protective Immunity to Limit Pulmonary and Extra-Pulmonary Bacterial Burden in Mice

doi: 10.3390/vaccines5040047

Figure Lengend Snippet: Sublingual immunization with Mycobacterium tuberculosis (Mtb) proteins Ag85B and ESAT-6 in the presence of alpha-galactosylceramide (α-GalCer) adjuvant induces systemic and mucosal T cell responses. Separate groups of mice ( n = 4–6) were immunized by sublingual route with one or two doses of mixture of Mtb proteins Ag85B and ESAT-6 in the presence or absence of α-GalCer adjuvant (w/aGC and w/o aGC, respectively) at 7 day intervals and sacrificed 7 days after each immunization to determine immune responses. Single cell suspensions from spleen and lung tissues were analyzed for IFN-γ producing cells specific for ESAT-6 (Panels ( A , B )) and Ag85B (Panels ( C , D )) using a standard IFN-γ ELISpot assay. Responses specific to the Mtb proteins were determined by subtracting the background values of medium stimulation from that of the individual protein stimulation and the mean ± S.D values were expressed as spot forming cells (SFC) per 10 5 input cells. Significant responses ( p < 0.05) between groups vaccinated in the presence and absence of α-GalCer (*) and between one and two doses (#) of vaccine delivered were noted. The experiment was performed using 5 mice/group and repeated 2 times.

Article Snippet: Three weeks after final immunization, the animals were infected with Mycobacterium tuberculosis Erdman (ATCC 35801) by the aerosol route (100 CFU/mouse through Glas-Col apparatus) as described previously [ ].

Techniques: Adjuvant, Enzyme-linked Immunospot

(A) Induction of autophagy in BMMs by ambroxol as determined by LC3B puncta. (B) Induction of autophagy in BMMs by ambroxol as determined by TFEB nuclear translocation. (C) Effects of ambroxol on M. tuberculosis Erdman survival in free culture and when infected into BMMs. Data are means ± SEM of 3 or 4 independent replicates. Tukey's multiple comparison: *, P < 0.05; **, P < 0.01; †, not significant. (D) Effect of ambroxol on autophagy in lung tissue. n = 2 mice/group. Data are means ± SEM.

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Ambroxol Induces Autophagy and Potentiates Rifampin Antimycobacterial Activity

doi: 10.1128/AAC.01019-18

Figure Lengend Snippet: (A) Induction of autophagy in BMMs by ambroxol as determined by LC3B puncta. (B) Induction of autophagy in BMMs by ambroxol as determined by TFEB nuclear translocation. (C) Effects of ambroxol on M. tuberculosis Erdman survival in free culture and when infected into BMMs. Data are means ± SEM of 3 or 4 independent replicates. Tukey's multiple comparison: *, P < 0.05; **, P < 0.01; †, not significant. (D) Effect of ambroxol on autophagy in lung tissue. n = 2 mice/group. Data are means ± SEM.

Article Snippet: M. tuberculosis Erdman (ATCC 35891) isolates were cultured in 7H10 medium with oleic acid-albumin-dextrose-catalase supplement, and bone marrow-derived macrophages (BMMs) were cultured in Dulbecco's modified Eagle's medium with 10% fetal bovine serum, 10 mM HEPES, and 1 mM pyruvate.

Techniques: Translocation Assay, Infection, Comparison